Assay development in high throughput screening - 2 ECTS

Course organised by LCBU and gives a practical basis for assay development in high throughput screening and is given as a practical complement to the course “Introduction to high throughput screening”.

Date: Feb 5-9, 2018

Venue: Laboratories of Chemical Biology Umeå (LCBU), Umeå University, Umeå

Application open: November 13, 2017
Application deadline: January 21, 2018

Responsible teacher/s:
Stina Berglund and Mikael Elofsson
Department of Chemistry, Umeå University

Course content
The course gives a practical basis for assay development in high throughput screening and is given as a practical complement to the course “Introduction to high throughput screening”. High throughput screening (HTS) is a technique used to identify biologically active small organic molecules through screening of compound libraries with the help of robust test systems and advanced instrumentation. The course includes several aspects of assay development applicable to both target-based and cell-based assays, however, the focus will be on cell-based assays. The assay carried out in the course includes cell culturing and bacterial infection, thus experience in cell culturing and good microbial lab practice is required. Parameters of importance for optimization of a cell based assay will be tested empirically in the laboratory and their significance calculated using Z'. During the course a small-scale screen will be executed. The students will thereby be given knowledge about the importance of good screening logistics and gain insight into the amount of data generated and handling thereof. During the course the students will be introduced to instrumentation commonly used in high throughput screening such as liquid handling instrumentation, plate readers and high content screening microscope.

Entry requirements

  • The theoretical web-based course “Introduction to high throughput screening” (2 ECTS).
  • Bachelor degree (120 ECTS) in chemistry, molecular biology, pharmacology or equivalent.
  • Practical experience in cell culturing and good microbial practice.
  • English knowledge corresponding to the Swedish upper secondary education English B.

For more information and registration: http://www.chemistry.umu.se/english/research/infrastructure/lcbu/training-and-education

Contact information for questions regarding the course and registration: stina.berglund01@umu.se


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