Measuring Metabolic Engines and Fuels with Agilent Seahorse XF Analyzer

Date: 2018-01-30—2018-01-31
Time: 1:00 PM
Location: NUS 6A–L - Biomedicinhuset, Department of Pharmacology and Neuroscience
The event is for: students - employees

In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes. Using the Seahorse XF Analyzer you can:
• Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand.
• Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.
• Identify fuel dependencies to uncover cancer cell vulnerabilities.
• Explore how fuel preferences lead to cell fate decisions for differentiation
and immune cell activation.

The workshop includes presentations by:

30 January:
13.00 – 14.00
Seahorse Intro Presentation
- Nick Howe, Agilent Technologies

14.00 – 14.25
Jonathan Gilthorpe

Department Pharmacology and Clinical Neuroscience
Optimisation of cell lines for Seahorse assays

14.25 – 14.45
Carl Herdenberg
Department of Radiation Sciences, Oncology
Phenotype analysis of Lrig-null mouse embryonic fibroblasts

14.45 – 15.05
Simon Tuck
Umeå Centre for Molecular Medicine
Metabolic rewiring in Caenorhabditis elegans

15.05 – 15.30
Susan Yellagunda
Department of Clinical Microbiology, Immunology
Investigating pathogen-host responses in human neutrophils

15.30 – 15.50
Jonathan Gilthorpe
Combining Seahorse and MS-based metabolomics to understand metabolic reprogramming in ALS

Wednesday, Jan 31, 2018
09.00 – 14.00
Wet lab session.
Hands on experience for Seahorse beginners. Limited spaces, registration required.

More information and registration:
Nick Howe,



Organizer: Department of Pharmacology and Clinical Neurosciences
Contact person: Jonathan Gilthorpe
Event type: Workshop
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