Measuring Metabolic Engines and Fuels with Agilent Seahorse XF Analyzer
Time: 1:00 PM
Location: NUS 6A–L - Biomedicinhuset, Department of Pharmacology and Neuroscience
The event is for: students - employees
In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes. Using the Seahorse XF Analyzer you can:
• Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand.
• Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.
• Identify fuel dependencies to uncover cancer cell vulnerabilities.
• Explore how fuel preferences lead to cell fate decisions for differentiation
and immune cell activation.
The workshop includes presentations by:
13.00 – 14.00
Seahorse Intro Presentation
- Nick Howe, Agilent Technologies
14.00 – 14.25
Department Pharmacology and Clinical Neuroscience
Optimisation of cell lines for Seahorse assays
14.25 – 14.45
Department of Radiation Sciences, Oncology
Phenotype analysis of Lrig-null mouse embryonic fibroblasts
14.45 – 15.05
Umeå Centre for Molecular Medicine
Metabolic rewiring in Caenorhabditis elegans
15.05 – 15.30
Department of Clinical Microbiology, Immunology
Investigating pathogen-host responses in human neutrophils
15.30 – 15.50
Combining Seahorse and MS-based metabolomics to understand metabolic reprogramming in ALS
Wednesday, Jan 31, 2018
09.00 – 14.00
Wet lab session.
Hands on experience for Seahorse beginners. Limited spaces, registration required.
More information and registration:
Nick Howe, email@example.com
Seminar Flying in the face of Cancer
Dissertation Behzad Khoshnood - molecular biology